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fitc-conjugated isolectin b 4  (Thermo Fisher)


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    Structured Review

    Thermo Fisher fitc-conjugated isolectin b 4
    Fitc Conjugated Isolectin B 4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/isolectin+b+4/fitc+conjugated+isolectin+b+4/pmc12219841-148-82-88
    Average 90 stars, based on 1 article reviews
    fitc-conjugated isolectin b 4 - by Bioz Stars, 2026-11
    90/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Genetic deficiency and pharmacological modulation of RORα regulate laser-induced choroidal neovascularization
    Article Snippet: .. Seven (7) days post-laser, CNV was analyzed in choroidal flat mounts with isolectin B 4 (Invitrogen, I21413) staining to visualize and quantify lesion size. ..

    Article Title: MicroRNA-143 plays a protective role in ischemia-induced retinal neovascularization
    Article Snippet: An oxygen controller (PROOX 110; BioSpherix, Parish, NY, USA) was used to monitor and control the oxygen level. .. The rats were sacrificed at P18, and their retinas were dissected and stained with isolectin B 4 (5 g/mL, Alexa Fluor 488, catalogue no. I21411; Life Technologies Australia, Mulgrave, VIC, Australia). ..

    Article Title: Analysis of microglial production of reactive oxygen and nitrogen species.
    Article Snippet: Reactive oxygen and nitrogen species are both regulators and effectors of microglial activation, and assays of these oxidants can be used as a measure of acute and chronic activation of microglial cells.. Here we describe quick methods to assess the production of superoxide, hydrogen peroxide, nitric oxide, and peroxynitrite by microglia.

    Article Title: Ephrin-A5 Is Involved in Retinal Neovascularization in a Mouse Model of Oxygen-Induced Retinopathy
    Article Snippet: .. Immunofluorescence staining was performed as follows: sections were permeabilized in PBS containing 0.1% Triton X-100 for 30 minutes, incubated in mouse anti-Efna5 mAb (1 : 100, ab60705, abcam, Cambridge, UK) and Isolectin-B 4 (1 : 100, I21411, Invitrogen, Carlsbad, CA, USA) overnight at 4°C. .. Immunofluorescence staining was performed as follows: sections were permeabilized in PBS containing 0.1% Triton X-100 for 30 minutes, incubated in mouse anti-Efna5 mAb (1 : 100, ab60705, abcam, Cambridge, UK) and Isolectin-B 4 (1 : 100, I21411, Invitrogen, Carlsbad, CA, USA) overnight at 4°C.

    Article Title: Postnatal Weight Gain Modifies Severity and Functional Outcome of Oxygen-Induced Proliferative Retinopathy
    Article Snippet: .. Retinas were dissected and stained overnight with fluoresceinated Isolectin B 4 (Alexa Fluor 594 – {"type":"entrez-nucleotide","attrs":{"text":"I21413","term_id":"1601767","term_text":"I21413"}} I21413 ; Molecular Probes, Eugene, OR) in 1 mmol/L CaCl 2 in PBS. .. Lectin-stained retinas were flat-mounted with the photoreceptor side down, and 20 images of each flat-mounted retina were obtained at 5× magnification on a Zeiss AxioObserver.Z1 microscope and merged to form one image using AxioVision 4.6.3.0 software.

    Immunostaining:

    Article Title: Analysis of microglial production of reactive oxygen and nitrogen species.
    Article Snippet: Reactive oxygen and nitrogen species are both regulators and effectors of microglial activation, and assays of these oxidants can be used as a measure of acute and chronic activation of microglial cells.. Here we describe quick methods to assess the production of superoxide, hydrogen peroxide, nitric oxide, and peroxynitrite by microglia.

    Membrane:

    Article Title: Amyloid β oligomers constrict human capillaries in Alzheimer’s disease via signaling to pericytes
    Article Snippet: .. All extracellular solutions contained isolectin B 4 ( 41 ) to label the basement membrane (ThermoFisher {"type":"entrez-nucleotide","attrs":{"text":"I21411","term_id":"1601765","term_text":"I21411"}} I21411 , 10 μg/ml), and hence to label pericytes which are enveloped by this , and 7.5 μM propidium iodide to label cells with membranes that had become non-specifically permeable ( 19 ). .. The Aβ oligomeric preparations were analysed via SDS-PAGE using 10-20% tris-glycine gels (EC61352BOX, Invitrogen).

    Article Title: Capillary pericytes mediate coronary no-reflow after myocardial ischaemia
    Article Snippet: .. Pericytes were labelled by expression of DsRed under control of the NG2 promoter (in mice), or with anti-NG2 (Merck Millipore AB5320 1:200 or AbCam ab50009 1:200) or anti-PDGFRβ (Santa Cruz Biotechnology sc-432 1:200) antibodies (in rats), and the capillary basement membrane was labelled with isolectin B 4 congugated to Alexa Fluor 647 (Molecular Probes, I32450) or FITC (Sigma-Aldrich, UK, L2895) as described ( ). .. Pericyte association with sympathetic terminals was imaged using antibody to tyrosine hydroxylase (Merck Millipore AB1542 1:500).

    Immunofluorescence:

    Article Title: Ephrin-A5 Is Involved in Retinal Neovascularization in a Mouse Model of Oxygen-Induced Retinopathy
    Article Snippet: .. Immunofluorescence staining was performed as follows: sections were permeabilized in PBS containing 0.1% Triton X-100 for 30 minutes, incubated in mouse anti-Efna5 mAb (1 : 100, ab60705, abcam, Cambridge, UK) and Isolectin-B 4 (1 : 100, I21411, Invitrogen, Carlsbad, CA, USA) overnight at 4°C. .. Immunofluorescence staining was performed as follows: sections were permeabilized in PBS containing 0.1% Triton X-100 for 30 minutes, incubated in mouse anti-Efna5 mAb (1 : 100, ab60705, abcam, Cambridge, UK) and Isolectin-B 4 (1 : 100, I21411, Invitrogen, Carlsbad, CA, USA) overnight at 4°C.

    Incubation:

    Article Title: Ephrin-A5 Is Involved in Retinal Neovascularization in a Mouse Model of Oxygen-Induced Retinopathy
    Article Snippet: .. Immunofluorescence staining was performed as follows: sections were permeabilized in PBS containing 0.1% Triton X-100 for 30 minutes, incubated in mouse anti-Efna5 mAb (1 : 100, ab60705, abcam, Cambridge, UK) and Isolectin-B 4 (1 : 100, I21411, Invitrogen, Carlsbad, CA, USA) overnight at 4°C. .. Immunofluorescence staining was performed as follows: sections were permeabilized in PBS containing 0.1% Triton X-100 for 30 minutes, incubated in mouse anti-Efna5 mAb (1 : 100, ab60705, abcam, Cambridge, UK) and Isolectin-B 4 (1 : 100, I21411, Invitrogen, Carlsbad, CA, USA) overnight at 4°C.

    Expressing:

    Article Title: Capillary pericytes mediate coronary no-reflow after myocardial ischaemia
    Article Snippet: .. Pericytes were labelled by expression of DsRed under control of the NG2 promoter (in mice), or with anti-NG2 (Merck Millipore AB5320 1:200 or AbCam ab50009 1:200) or anti-PDGFRβ (Santa Cruz Biotechnology sc-432 1:200) antibodies (in rats), and the capillary basement membrane was labelled with isolectin B 4 congugated to Alexa Fluor 647 (Molecular Probes, I32450) or FITC (Sigma-Aldrich, UK, L2895) as described ( ). .. Pericyte association with sympathetic terminals was imaged using antibody to tyrosine hydroxylase (Merck Millipore AB1542 1:500).

    Control:

    Article Title: Capillary pericytes mediate coronary no-reflow after myocardial ischaemia
    Article Snippet: .. Pericytes were labelled by expression of DsRed under control of the NG2 promoter (in mice), or with anti-NG2 (Merck Millipore AB5320 1:200 or AbCam ab50009 1:200) or anti-PDGFRβ (Santa Cruz Biotechnology sc-432 1:200) antibodies (in rats), and the capillary basement membrane was labelled with isolectin B 4 congugated to Alexa Fluor 647 (Molecular Probes, I32450) or FITC (Sigma-Aldrich, UK, L2895) as described ( ). .. Pericyte association with sympathetic terminals was imaged using antibody to tyrosine hydroxylase (Merck Millipore AB1542 1:500).



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    Double fluorescence of the GRP (green) and superficial layers markers (magenta) of Sp5C and cervical spinal dorsal horn in adult macaque monkeys. Many GRP-ir fibers were colocalized with the marker for peptidergic neurons CGRP (a–c) and substance P (d–f) in the Sp5C. GRP-ir fibers were not colocalized with the marker for nonpeptidergic neurons <t>IB</t> <t>4</t> in the Sp5C (g–i). Most GRP-ir fibers were colocalized with CGRP(j–l) and substance P (m–o) in the superficial layers of the cervical spinal cord. GRP-ir fibers were not colocalized with IB 4 (p–r) in the cervical spinal dorsal horn. Bars = 200 μm (i, r)
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    Image Search Results


    Timp2 , Timp3 , and Timp4 are highly expressed in slowly dividing NPCs. (A) The scheme for isolating rapidly dividing and slowly dividing NPCs. H2B-GFP was transiently induced at E9.5 and the LGE was dissected at E17.5. NPCs were defined as cells positive for NPC marker CD133, negative for neuronal marker CD24, and negative for endothelial marker Isolectin B 4 . (B) Representative flow cytometric histogram of H2B-GFP fluorescence intensity. Rapidly dividing NPCs (bottom 10% of NPCs for H2B-GFP intensity) and slowly dividing NPCs (top 10% of NPCs for H2B-GFP intensity) were collected. (C) Quantitative RT-PCR analysis of Timp2 , Timp3 , and Timp4 mRNAs. Data are means ± SEM ( n = 3 independent experiments). * p < 0.05 by two-tailed paired t test.

    Journal: Frontiers in Neuroscience

    Article Title: TIMP3 promotes the maintenance of neural stem-progenitor cells in the mouse subventricular zone

    doi: 10.3389/fnins.2023.1149603

    Figure Lengend Snippet: Timp2 , Timp3 , and Timp4 are highly expressed in slowly dividing NPCs. (A) The scheme for isolating rapidly dividing and slowly dividing NPCs. H2B-GFP was transiently induced at E9.5 and the LGE was dissected at E17.5. NPCs were defined as cells positive for NPC marker CD133, negative for neuronal marker CD24, and negative for endothelial marker Isolectin B 4 . (B) Representative flow cytometric histogram of H2B-GFP fluorescence intensity. Rapidly dividing NPCs (bottom 10% of NPCs for H2B-GFP intensity) and slowly dividing NPCs (top 10% of NPCs for H2B-GFP intensity) were collected. (C) Quantitative RT-PCR analysis of Timp2 , Timp3 , and Timp4 mRNAs. Data are means ± SEM ( n = 3 independent experiments). * p < 0.05 by two-tailed paired t test.

    Article Snippet: Dissociated cells were incubated first for 15 min on ice in 0.2% bovine serum albumin (BSA)/phosphate-buffered saline (PBS) with primary antibodies—PE/Cy7 anti-CD133 (1:100, BioLegend Cat# 141210, RRID:AB_2564069), APC anti-CD24 (1,100, BioLegend Cat# 101814, RRID:AB_439716), and anti-Isolectin B 4 (biotin conjugate) (1,1,000, Sigma-Aldrich Cat# L2140, RRID:AB_2313663)—and then for 5 min on ice in 0.2% BSA/PBS with Streptavidin PE (1,500, eBioscience Cat# 12-4317-87).

    Techniques: Marker, Fluorescence, Quantitative RT-PCR, Two Tailed Test

    Double fluorescence of the GRP (green) and superficial layers markers (magenta) of Sp5C and cervical spinal dorsal horn in adult macaque monkeys. Many GRP-ir fibers were colocalized with the marker for peptidergic neurons CGRP (a–c) and substance P (d–f) in the Sp5C. GRP-ir fibers were not colocalized with the marker for nonpeptidergic neurons IB 4 in the Sp5C (g–i). Most GRP-ir fibers were colocalized with CGRP(j–l) and substance P (m–o) in the superficial layers of the cervical spinal cord. GRP-ir fibers were not colocalized with IB 4 (p–r) in the cervical spinal dorsal horn. Bars = 200 μm (i, r)

    Journal: The Journal of comparative neurology

    Article Title: Characterization of the expression of gastrin-releasing peptide and its receptor in the trigeminal and spinal somatosensory systems of Japanese macaque monkeys: Insight into humans

    doi: 10.1002/cne.25376

    Figure Lengend Snippet: Double fluorescence of the GRP (green) and superficial layers markers (magenta) of Sp5C and cervical spinal dorsal horn in adult macaque monkeys. Many GRP-ir fibers were colocalized with the marker for peptidergic neurons CGRP (a–c) and substance P (d–f) in the Sp5C. GRP-ir fibers were not colocalized with the marker for nonpeptidergic neurons IB 4 in the Sp5C (g–i). Most GRP-ir fibers were colocalized with CGRP(j–l) and substance P (m–o) in the superficial layers of the cervical spinal cord. GRP-ir fibers were not colocalized with IB 4 (p–r) in the cervical spinal dorsal horn. Bars = 200 μm (i, r)

    Article Snippet: Double-fluorescence staining of GRP and biotin-conjugated isolectin B 4 (IB 4 ) (1:1,000; cat. no. 121414; Molecular Probes, Eugene, OR) was also performed.

    Techniques: Fluorescence, Marker